AC Diagnostics and Nano Diagnostics
About the Company
At Boston Mountain Biotech, we develop and patent technology that aids in the development of a new protein expression and purification platform. Currently this technology is being marketed through the Lotus® E. coli Cell Lines which demonstrate reduced host cell protein (HCP). These new reduced HCP bacterial cell lines simplify the development and manufacturing of recombinant protein therapeutics, vaccines and enzymes. At BMB, we are revolutionizing the pharmaceutical industry by providing protein purification solutions that result in new drugs moving from development to patient faster while simultaneously lowering the cost of manufacturing.
Official Company Website
Official Company Website
Contract Research Services
Lotus™ Cell Lines Boston Mountain Biotech has developed the patent pending Lotus™ cell lines that offer improved downstream purification of recombinant protein products without the reliance on affinity tags or costly resins. Currently, Lotus™ has been optimized for use with ion exchange (IEX) based chromatography but we are continuing to expand the cell line suite into other resins common to the industry. To support the use of these cell lines BMB offers services in conjunction with the sale of these cell lines to further provide integration with the client’s existing processes.
Molecular Biology and Fermentation Services, including HCD (high cell density)In addition to our Lotus™ product offerings, Boston Mountain Biotech is a contract research organization (CRO) specializing in recombinant protein expression. We are equipped to handle production in bacterial, yeast, and insect cell systems.
Protein Purification ServicesBoston Mountain Biotech offers protein services to customers who already have a construct or are using our Lotus™ cell line. BMB is outfitted with downstream equipment used to produce both intracellular and secreted biologicals.
- Comparative utilization studies
- BMB will express and purify the client’s desired recombinant protein in both the Lotus™ cell line as well as a cell line of their choice
- Data will be provided comparing the two cell lines in the areas of growth, expression and purification.
- Additional cell line improvements
- BMB will provide additional modifications to the existing Lotus™ cell lines or design a fully customized Lotus™ cell line based upon the client’s specific needs
- Data analytic services can be provided to the client to guide rational choices that support efficient growth, expression and purification methodology
Molecular Biology and Fermentation Services, including HCD (high cell density)In addition to our Lotus™ product offerings, Boston Mountain Biotech is a contract research organization (CRO) specializing in recombinant protein expression. We are equipped to handle production in bacterial, yeast, and insect cell systems.
- Plasmid construction, isolation, and transformation
- Homologous recombination
- Expression studies
- Medium formulation and optimization
- High cell density (fed batch) fermentation – Applikon® platforms
Protein Purification ServicesBoston Mountain Biotech offers protein services to customers who already have a construct or are using our Lotus™ cell line. BMB is outfitted with downstream equipment used to produce both intracellular and secreted biologicals.
- Column selection and purification scheme design
- Custom purification and purification optimization – ATKA® and Millipore® platforms
- Post processing – tag cleavage,
- Analytics, including mass spectroscopy, ELISA, SDS PAGE and Western blot analysis